ATP-driven sodium pump in Streptococcus faecalis.

Sodium/proton antiporter in Streptococcus faecalis.

Streptococcus faecalis, like other bacteria, accumulates potassium ions and expels sodium ions. This paper is concerned with the pathway of sodium extrusion. Earlier studies (D.L. Heefner and F.M. Harold, Proc. Natl. Acad. Sci. USA 79:2798-2802, 1982) showed that sodium extrusion is effected by a primary, ATP-linked sodium pump. I report here that cells grown under conditions in which sodium AT...

ATP-linked sodium transport in Streptococcus faecalis. II. Energy coupling in everted membrane vesicles.

Everted membrane vesicles of Streptococcus faecalis accumulated “Na+ to a nominal concentration gradient of about 100 when incubated with ATP and M&+. The characteristics of “Na+ accumulation varied in a complex manner as a function of the pH and of the ionic environment. To illustrate, when the vesicles were prepared in potassium ma1eate:Hepes:sucrose buffer (0.45 N K+) and incubated in the sa...

Hydroxylysine metabolism in Streptococcus faecalis.

aureus, and Streptococcus faecalis

SYNOPSIS The incidence of trimethoprim resistance in coliforms and multiresistant strains of Staphylococcus aureus isolated in Bristol from 1970 to 1972 is low-2-3 and 1 0% respectively. The resistance is probably intrinsic; there is no evidence that it is R-factor or plasmid mediated. A single mechanism that confers resistance to both trimethoprim and sulphamethoxazole has not been detected. N...

Phospholipids of Streptococcus faecalis.

Autoradiograms of total lipid extracts from Streptococcus faecalis ATCC 9790, harvested in the stationary phase from a medium containing (32)P-orthophosphate, showed six major spots. The corresponding compounds were identified as diphosphatidylglycerol (possibly with a penta acyl structure); phosphatidylglycerol; a provisionally identified mixture of alanylphosphatidylglycerol and of the 2'-lys...